Cloning, heterologous expression, and characterization of the Erysipelothrix rhusiopathiae DnaK protein
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چکیده
منابع مشابه
Solubilization and Characterization of a Protective Antigen of Erysipelothrix rhusiopathiae.
A particulate fraction of Erysipelothrix rhusiopathiae cultures has been subjected to butanol extraction and treatment with surface-active agents in an attempt to solubilize a protective antigen. The particulate fraction was partitioned into the butanol layer but was not solubilized. Only sodium dodecyl sulfate solubilized the particles. The soluble protective activity was not sedimented by cen...
متن کاملCloning and expression in Escherichia coli of a protective antigen of Erysipelothrix rhusiopathiae.
Erysipelothrix rhusiopathiae is a primary pathogen of swine and turkeys and sporadic cause of disease in a variety of other hosts, including humans. A genomic library of the highly virulent strain of E. rhusiopathiae E1-6P was constructed in the expression-cloning vector lambda gt11 and screened with serum from a pig convalescent from an E. rhusiopathiae experimental infection. Immunoreactive c...
متن کاملMolecular cloning, expression, and characterization of dnaK in Streptococcus pneumoniae.
DnaK is known to be highly conserved in all species and is a major immunogen in Streptococcus pneumoniae. To elucidate the role of dnaK in S. pneumoniae, dnaK was cloned in Escherichia coli using a homologous dnaK probe generated by PCR. The His-tagged DnaK was overexpressed in soluble form and purified from E. coli. Alignment of the deduced DnaK amino acid sequence from nucleotide sequences of...
متن کاملMechanism of plasma clotting by Erysipelothrix rhusiopathiae.
Experiments were performed to determine the mechanism by which Erysipelothrix rhusiopathiae clots plasma. Detection of plasma-clotting activity in four strains of E. rhusiopathiae was carried out by mixing a 24-h broth culture of a tested bacterial strain with rabbit plasma (tube coagulation test). Sodium citrate, sodium oxalate, EDTA, and heparin were used as anticoagulants in preparing the ra...
متن کاملPotential errors in recognition of Erysipelothrix rhusiopathiae.
Here we describe four isolations of Erysipelothrix rhusiopathiae associated with polyarthralgia and renal failure, septic arthritis, classic erysipeloid, and peritonitis. Although the biochemical identification was straightforward in each case, recognition presented a challenge to the clinical microbiologist, since in three cases E. rhusiopathiae was not initially considered due to unusual clin...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1993
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.61.2.411-417.1993